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Genotoxicology
The Genotoxicology team
works in a purpose-built suite of
laboratories and offers a wide range of genetic toxicology and cell
biology tests.
We are able to offer a
comprehensive range of genetic toxicity studies including the
complete standard battery of tests for preclinical safety
assessment, a range of studies suitable for early screening of
candidate compounds and various in-vitro models of toxicity and
local tolerance.
Mutagenicity
Bacterial Reverse
Mutation Test (Ames
Test)
- Plate incorporation
- Pre-incubation
- Screening for early
candidate development
Gene Mutation in
Mammalian Cells
- L5178Y TKą (Mouse
Lymphoma Assay) in microwell plates
- CHO, V79 HGPRT locus
- Screening for early candidate
development
Chromosome
aberration
- Human lymphocytes
(HL)
- Chinese Hamster
Ovary cells (CHO)
- Screening for early
candidate development
In Vitro Rodent Micronucleus test
-
Mouse bone marrow
-
Rat bone marrow
Cell Biology
Cytotoxicity
- Variety of endpoints
- SRB
- MTT
- Colony-forming
capacity
- Microscopic
examination
Haemolysis
Validation Work
Following approval of the OECD guideline, the Genotoxicology team
will validate the
in vitro micronucleus assay, an assay which concurrently
measures cytotoxicity (using the cytokinesis-block proliferation
index) and genotoxicity (measurement of the frequency of
micronucleated binucleate cells). This assay will be invaluable as a
high throughput screening tool, or as one of the battery of
in vitro mammalian regulatory assays that Sequani
provide, in order to screen test compounds for clastogenic and
aneugenic potential.
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